'March on Wall Street South' draws hundreds

Chuck Burton / AP

Demonstrators gather at Frazier Park at the start of a protest march Sunday in Charlotte, N.C.

By NBC News staff

Updated at 3:18 p.m. ET: About 800 people chanting and carrying signs (among them, "Banks got bailed out. We got sold out") marched Sunday through the central business district in Charlotte, N.C., ahead of the Democratic National Convention to protest what they said was seedy corporate influence on politics.

The protesters, who came from across the country, gathered at Frazier Park for a round of speeches before starting the march. They?were from a coalition of more than 80 local and national groups.?Media packets declared the group's mission as "building peoples' power during the DNC.

Demonstrators had signs indicating that some were union workers, anti-war veterans and undocumented immigrants.?

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"Capitalism is holding back the human race," one sign read. "Bail out people, not banks," another sign said.

Hundreds of chanting protesters march through uptown Charlotte, N.C., ahead of the Democratic National Convention. WCNC reports.

The protest route was to take them past the corporate headquarters of Bank of America and a major office hub for Wells Fargo ? financial institutions that some see as symbols of foreclosures and federal bailouts. Protesters also planned a stop at the headquarters of Duke Energy, the nation?s largest utility.

Duke has been criticized for investing in nuclear energy and using coal, according to the Charlotte Observer. Protesters have asked the company to focus on wind and solar power, which emit less carbon into the atmosphere. Duke, in defense, says it aims to retire its older coal plants.

Uniformed police officers on bicycles and on foot kept a close eye on the marchers. Ray Suarez, of PBS NewsHour, tweeted, "Just watched Occupy et. al. march through Charlotte, who were in turn watched by enough cops to invade a small country."

Before the main event began, a small press conference was held highlighting a woman, Sylvia Sanchez, who says she must pay $20,000 to Bank of America by Sept. 14 to avoid foreclosure on a home she's owned for 12 years.?The woman, whose daughter is suffering from a brain condition called hydrocephalus, said she is in debt after having retrofitted the home with wheelchair ramps.

In interviews with NBC News, rank-and file-activists cited different reasons for coming.

Kendall Hale, a massage therapist and Obama campaign volunteer from Asheville, N.C., said that climate change was among her concerns.

"The era of compromise may not be working so well," Hale said. She urged President Obama to "forge ahead with the issues [he's] speaking about so eloquently."

Andie Marion, a college student also from the Asheville area, said her chief concern was "money in politics."

"The amount of corporate power that influences a lot of the politics I think is really huge," she said.

On the sidelines, Sunday's protest in Charlotte triggered a conversation about where the Occupy movement is headed.?

Todd Gitlin, who wrote Letters to a Young Activist and Occupy Nation: The Roots, the Spirit, and the promise of Occupy Wall Street, told NPR: "The initial wave moved its metaphors into the household conversation and shaped politics. But I think they essentially reached the limits of what they could achieve. Now there's a sorting process."

The Democratic National Convention opens Tuesday in Charlotte's Time Warner Cable Arena. President Barack Obama will be nominated for a new term on Wednesday. He will deliver his acceptance speech Thursday night at the outdoor Bank of America Stadium.

The convention starts less than a week after Republicans gathered in Tampa, Fla., to nominate Mitt Romney as the party's presidential candidate.

NBC's Jamie Novogrod and Mark Potter and contributed to this report.

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Source: http://usnews.nbcnews.com/_news/2012/09/02/13621900-hundreds-take-part-in-march-on-wall-street-south-in-charlotte-nc?lite

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Scientists probe mystery of operon evolution

Saturday, September 1, 2012

The threads of an evolutionary mystery that dates to the birth of molecular biology are beginning to unravel, thanks to a new investigation by computational bioengineers at Rice University and the University of Texas MD Anderson Cancer Center.

In new research published online this week in PLOS Computational Biology, Rice's Oleg Igoshin and MD Anderson's Christian Ray offer a possible explanation for the existence of jointly controlled clusters of genes called operons, which are found in bacterial chromosomes but not in those of higher order organisms like humans.

The new study harkens to one of the earliest 20th-century discoveries in molecular biology, and it could help clear 21st-century hurdles for synthetic biologists.

In the early 1960s, just as scientists were discovering how cells transcribed information from DNA to create the necessary proteins for life, French scientists Jacques Monod and Fran?ois Jacob found that the bacterium Escherichia coli used three specialized genes to create the proteins it needed to break down and digest lactose. They also found that these three metabolic genes were switched on and off together from a single control point.

Monod and Jacob had discovered the first operon, a set of multiple genes that are controlled as one. It marked the first time that scientists had identified a gene regulatory network, and it earned them a share of the 1965 Nobel Prize in Physiology or Medicine. Monod and Jacob's lac operon turned out to be the first of many bacterial operons. But by the late 1960s, it was clear that operons weren't the biological norm. None have ever been found in humans, for instance, and very few have been identified in multicellular organisms.

"There's never been a definitive explanation for why nature would preferentially select for operons in prokaryotes but not in eukaryotes," said Igoshin, associate professor of bioengineering at Rice. "In addition, we do not know how genes get grouped into operons. Why, for example, are some interacting genes selected to be in one operon while others are not?"

Igoshin and Ray are computational bioengineers who apply mathematics and computational bioinformatics to study cell signaling and other biochemical processes. Ray, a former Rice postdoctoral research associate in Igoshin's research group, is now in the Department of Systems Biology at MD Anderson. Ray and Igoshin began investigating operons in late 2009 to determine whether their evolution might have been influenced by the "noisy" nature of biochemical signals that regulate bacterial gene transcription.

"When a cell is responding to its environment, it can use regulators to control gene expression, but the amount of control that the cell has is limited by the number of molecules like messenger RNAs, which mediate protein production," Ray said. "So, in bacteria, the number of copies of a protein expressed by a gene can vary widely, say from 50 one hour to 100 the next. And this happens even when conditions outside the cell have not changed."

Igoshin and Ray knew that these random fluctuations were less of an issue for eukaryotic cells, which have larger volumes and more copies of messenger RNA and proteins. So they hypothesized that operons play a role in helping bacteria deal with these "noisy" conditions.

To test their idea, they developed a series of mathematical models of gene networks that could be run on a computer rather than on cell cultures, as well as statistical tests that could be performed using the information accumulated in bioinformatic databases.

Their mathematical models of gene networks covered six different types of protein-protein interactions. For each interaction type, they compared how operons affected noise in networks encoded by the member genes. For three of the six networks, operons worked to suppress noise. For the other three, they worked to increase noise. The findings from the simulations therefore suggested that operons could reduce the detrimental effects of noisy signals in some gene regulatory networks, but not others.

To further test the idea, Igoshin and Ray examined the operon organization of the E. coli genome. They found operons were frequent when the type of interaction that they encoded worked to suppress noise. When the encoded interaction did not suppress noise, operons were infrequent.

"Operons that emerged in the course of evolution in E. coli are consistent with selection for noise suppression and selection against noise amplification," Igoshin said.

The study also suggested why specific genes might be found in a specific operon.

"Certain genes perform much better when they're controlled as a unit, particularly if they produce co-ingredients that are required in proportional amounts," Ray said. "In the simulations, when these were split up and put onto separate operons, the inherent noise in the control signals would create a situation where the cell had way too much or too little of one co-product. In some cases, this is just inefficient, but in others the buildups could be toxic."

Ray and Igoshin said the study has implications for synthetic biologists who are trying to imbue cells with new biological functions not found in nature.

"For example, if you need to take multiple enzymes from different species and put them into a bacterium -- something that was done recently to produce a low-cost anti-malaria drug -- it might be easier to take them separately and put them into different parts of the chromosome," Igoshin said. "What this new finding shows is that there may be a cost for that in terms of overall fitness of the organism. Nature sometimes groups things together, particularly in cases where one of the enzymes makes or consumes toxic intermediates, and synthetic biologists would do well to pay attention to these types of interactions as they prepare their designs."

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Rice University: http://media.rice.edu

Thanks to Rice University for this article.

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Source: http://www.labspaces.net/123150/Scientists_probe_mystery_of_operon_evolution

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Kids Workshops to Learn, Create and Craft at The Home Depot ...

Free Kids Workshops to Learn, Create & Craft?at The Home Depot

Fall is a favorite time of year for many. I prefer summer, but I do enjoy a few aspects of fall. Crunchy leaves, before they get soggy, a fire in the fireplace, warm crockpot comfort meals, and yes FOOTBALL. ?Who doesn?t love football. I love both American and?European?football, but fall is for American football.

Home Depot is tapping this enthusiasm with a workshop this Saturday. ?Home Depot has a great series of workshops, not just for kids though, but for adults as well. ?From weatherizing your home to Do-It-Herself workshops. ?So, take advantage of the turning weather, take some free workshops and enjoy yourself.

Workshop Schedule

Saturday,?September 1st?at 9am. You can build a goal post football game.

Saturday, October 6th at 9am: Build a?fire truck

Mom of 3, Wife to 1, blogger, and CPA - Crazy life is a happy life around here. This entry was posted in Tacoma and tagged Build, Craft, Crafts, Hands on. Bookmark the permalink.

Source: http://funonadimewa.wordpress.com/2012/08/31/free-kids-workshops-to-learn-create-craft-at-the-home-depot/

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Five Worst Mistakes in Hiring a Moving Company

Some people are going to shift your residence to a new location with entire stuffs, most likely they will want services of an expert moving company for comfortable and smooth shifting of your valuable stuffs. But now a day several shipping agencies available in India and people can easily get them. But lots of time people do some common mistakes that result worst and make their shifting more complicated and time consuming.

Unregistered Moving Company Hiring unregistered moving company is a common mistake. Many people plan their move with unregistered shifting agencies to save some amount of money but it must be avoided. You should always prefer registered and reputed shifting agencies for relocation because your goods are important and their safe transportation is the prime issue.

Cheap Moving Services There are many moving agencies offer you services at very low rates in comparison to average market rates but you should avoid them. Most of the cheap rate agencies are not up to the standard. They are not able to provide you good quality and safe relocation services. They don't have proper staff and resources to serve you effectively.

No Investigation and Research Work Hiring moving company without proper research work is very common that can cause poor services and financial losses too. So, before hiring any moving company you should take detail information about the company's experience, service standards, expertise area and policies. Don?t take detail information over phone call. Personally visit the nearest branch of the company and meet from their representatives and staff members. Also take information about goods transporters and resources of the company.

Hiring Moving Company without Home Inspection Most of the expert packing and transportation service providers perform complete home inspection at least once before committing for their services. If any moving company is not interested in your home inspection, most probably the agency is not able to serve you effectively. You should avoid such kinds of service providers.

Uninsured Moving Company Planning shipping of goods with an uninsured moving is the most traumatic decision in any relocation. Transportation of goods to a new place involves lots of uncertainty and you should always plan your move with moving company that offer complete insurance coverage for the entire moving stuffs.

These are the seven worst and common mistakes that people do in hiring professional moving company. If you are going to relocate and looking for expert packers and movers, you must avoid these mistakes.

Trilok Singh Rawat is an experienced professional of transportation industry. He has worked with many reputed Packers and Movers Visakhapatnam based agencies. He also writes free articles over safe and trouble-free relocation tips. Now he is running own packers and movers based moving company and offers wide range of relocation services according to demands of people.

This article has been viewed 36 time(s).

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INTERNET MARKETING MAKE MONEY ONLINE NICHE BLOG ...

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